Cell Signaling Technology

Product Pathways - Phosphatases

PPP2R2A (2G9) Mouse mAb #5689

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 52 kDa Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PPP2R2A (2G9) Mouse mAb recognizes endogenous levels of total PPP2R2A/PP2A, B55-α/PR55-α protein. This antibody cross-reacts with overexpressed PPP2R4 and PPP2R5D proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp406 of rat PP2R2A/PP2A, B55-α/PR55-α protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PPP2R2A (2G9) Mouse mAb.

Background

Protein phosphatase 2A subunit B isoform R2-α (PPP2R2A) is the component of regulatory subunit B of serine/threonine protein phosphatase 2A (PP2A). PPP2R2A belongs to the R2/B55/PR55 family of regulatory subunit B (1). Evidence suggests that subunit PPP2R2A enhances TGF-β, Activin, and Nodal signaling pathways (7) and may play an important role in cell cycle regulation (2-4). PPP2R2A/PP2A, B55-α/PR55-α isoform interacts with the type I TGF-β receptors via unique WD-40 repeats (5) that contribute to the anti-proliferative function of TGF-β receptors (6).

  1. Li, H.H. et al. (2007) EMBO J 26, 402-11.
  2. Mayer-Jaekel, R.E. et al. (1993) Cell 72, 621-33.
  3. Clarke, P.R. et al. (1993) Mol Biol Cell 4, 397-411.
  4. Healy, A.M. et al. (1991) Mol Cell Biol 11, 5767-80.
  5. Neer, E.J. et al. (1994) Nature 371, 297-300.
  6. Griswold-Prenner, I. et al. (1998) Mol Cell Biol 18, 6595-604.
  7. Batut, J. et al. (2008) Development 135, 2927-37.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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