Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

LCMT1 (4A4) Mouse mAb #5691

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP M R Endogenous 39 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LCMT1 (4A4) Mouse mAb recognizes endogenous levels of total LCMT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to mouse LCMT1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, H2.35, and CTLL-2 cells using LCMT1 (4A4) Mouse mAb.

Background

Leucine carboxyl methyltransferase-1 (LCMT1) is an important mammalian methyltransferase that methylates the catalytic C subunit of protein phosphatase 2A (PP2A) at Leu309 (1,2). X-ray crystallography analysis reveals that the structural integrity of human LCMT1 and cofactor S-adenosylmethionine complex enables proper interaction with PP2A (3). Evidence suggests that functional LCMT1 is crucial for the ability of PP2A to form heterotrimers with the B/B55 and Bα family of regulatory subunits (4,5). Impaired LCMT1 activity is shown to disrupt mitosis and cell survival (5). In neurodegenerative models, increases in endogenous levels of LCMT1, methylated PP2A (C), and Bα induce the development and differentiation of neuroblastoma cells (5-7).

  1. Xie, H. and Clarke, S. (1994) Biochem Biophys Res Commun 203, 1710-5.
  2. Lee, J. and Stock, J. (1993) J Biol Chem 268, 19192-5.
  3. Tsai, M.L. et al. (2011) Acta Crystallogr D Biol Crystallogr 67, 14-24.
  4. Schild, A. et al. (2006) Int J Dev Neurosci 24, 437-43.
  5. Lee, J.A. and Pallas, D.C. (2007) J Biol Chem 282, 30974-84.
  6. Sontag, E. et al. (2004) J Neuropathol Exp Neurol 63, 1080-91.
  7. Sontag, J.M. et al. (2010) J Neurochem 115, 1455-65.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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