Product Pathways - Companion Products
Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate) #5698
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IP | Transfected Only | Mouse IgG2a |
Applications Key:
IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5698:
- Immunoprecipitation
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate) detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
IP
Immunoprecipitation of COS-7 cell extracts, untransfected or transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (Myc-CSL) protein, using Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate). Blots were probed with Myc-Tag (71D10) Rabbit mAb #2278.
Description
This Cell Signaling Technology antibody is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic bead. Myc-Tag (9B11) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of Myc epitope-tagged proteins.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
