Cell Signaling Technology

Product Pathways - Phosphatases

IGBP1 (5F6) Mouse mAb #5699

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 42 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IGBP1 (5F6) Mouse mAb recognizes endogenous levels of total IGBP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human IGBP1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IGBP1 (5F6) Mouse mAb.

Background

Immunoglobulin binding protein 1 (IGBP1) interacts with the regulatory subunit C of serine/threonine phosphatase PP2A, and other protein phosphotases, PP4 and PP6 (1-3). Binding of IGBP1 to PP2A has been shown to regulate PP2A catalytic activity and its substrate specificity (1-4). Recent evidence suggests that IGBP1 may play a role in PP2Ac ubiquitination via its association with E3 ubiquitin ligase MID1 (5,6). IGBP1 negatively regulates apoptosis by targeting PP2A activity to suppress p38 mitogen-activated protein kinase activation by cytokines (7). Upon BCR cross-linking, IGBP1 transiently associates with tyrosine phosphorylated molecules, which in turn induce downstream signal transduction (7). Evidence suggests that IGBP1 association with PP2A may be involved in the rapamycin sensitive mTOR pathway (8,9). Formation of the IGBP1 and Midline ring finger protein (MID) complex has been identified as a prerequisite to pathogenesis of X-linked Optiz GBBB syndrome (10).

  1. Prickett, T.D. and Brautigan, D.L. (2006) J Biol Chem 281, 30503-11.
  2. Prickett, T.D. and Brautigan, D.L. (2004) J Biol Chem 279, 38912-20.
  3. Chen, J. et al. (1998) Biochem Biophys Res Commun 247, 827-32.
  4. Sakashita, S. et al. (2011) Pathol Int 61, 130-7.
  5. McConnell, J.L. et al. (2010) Biochemistry 49, 1713-8.
  6. McDonald, W.J. et al. (2010) J Cell Biochem 110, 1123-9.
  7. Murata, K. et al. (1997) Proc Natl Acad Sci U S A 94, 10624-9.
  8. Prickett, T.D. and Brautigan, D.L. (2007) Mol Cell Biol 27, 4217-27.
  9. Inui, S. et al. (1998) Blood 92, 539-46.
  10. Short, K.M. et al. (2002) BMC Cell Biol 3, 1.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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