Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

WNK4 Antibody #5713

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Dg (B) Endogenous 135-140 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WNK4 Antibody detects endogenous levels of total WNK4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a sythetic peptide corresponding to residues surrounding Lys59 of human WNK4. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from G-361, 786-0 and MDCK cells using WNK4 Antibody.

Background

The WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four WNK family members have been identified in humans (WNK1-4) and have been implicated in regulating ion permeability (3). Mutations in the WNK1 and WNK4 genes in humans cause pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder leading to hypertension, hyperkalemia, and renal tubular acidosis (4). WNK4 is specifically expressed in the kidney, whereas WNK1 has a wider distribution but is predominantly expressed in polarized epithelia (1-3). Heterozygous mutations in WNK1 in mice result in a significant decrease in blood pressure, while homozygous mutations are embryonic lethal (5). WNK1 is phosphorylated by Akt at Thr60 (6). In addition, WNK1 may be autophosphorylated at Ser382 in the activation loop, and this is thought to be required for its kinase activity (7).

  1. Veríssimo, F. and Jordan, P. (2001) Oncogene 20, 5562-9.
  2. Xu, B. et al. (2000) J Biol Chem 275, 16795-801.
  3. Choate, K.A. et al. (2003) Proc Natl Acad Sci U S A 100, 663-8.
  4. Wilson, F.H. et al. (2001) Science 293, 1107-12.
  5. Zambrowicz, B.P. et al. (2003) Proc Natl Acad Sci U S A 100, 14109-14.
  6. Vitari, A.C. et al. (2004) Biochem J 378, 257-68.
  7. Xu, B.E. et al. (2002) J Biol Chem 277, 48456-62.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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