Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Neuroscience

CNPase (D83E10) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #5716

Applications Reactivity Sensitivity Isotype
IF-F H M R Endogenous Rabbit IgG

Applications Key:  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CNPase (D83E10) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total CNPase protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val81 of human CNPase protein.

IF-F

IF-F

Confocal immunofluorescent analysis of rat cerebellum using CNPase (D83E10) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor) and β3-Tubulin (TU-20) Mouse mAb #4466 (green). Red = Propiduim iodide (fluorescent DNA dye).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat tissue. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated CNPase (D83E10) XP® Rabbit mAb #5664.

Background

CNPase (2', 3’-cyclic nucleotide 3'-phosphodiesterase) catalyzes the in vitro hydrolysis of 2’, 3’-cyclic nucleotides to produce 2’-nucleotides. The in vivo molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4).

  1. Esposito, C. et al. (2008) Biochemistry 47, 308-19.
  2. Kozlov, G. et al. (2003) J Biol Chem 278, 46021-8.
  3. Lee, J. et al. (2005) J Cell Biol 170, 661-73.
  4. Lappe-Siefke, C. et al. (2003) Nat Genet 33, 366-74.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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