Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Cystatin C (hCystatin C) #5717

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Source

Recombinant human Cystatin C (hCystatin C) Ser27-Ala146 (Accession #NP_000090) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hCystatin C does not have a Met on the amino terminus and has a calculated MW of 13,347. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal SSPGK of recombinant hCystatin C was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCystatin C. All lots are greater than 98% pure.

Bioactivity

The activity of hCystatin C was determined by assessing its ability to inhibit papain-mediated cleavage of a fluorogenic subtrate. The ED50 of each lot 1-12 nM.

Coomassie Gel

Coomassie Gel

The purity of recombinant hCystatin C was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hCystatin C and staining overnight with Coomassie Blue.

Bioactivity

Bioactivity

The activity of hCystatin C was determined by assessing its ability to inhibit papain-mediated cleavage of a fluorogenic subtrate, Z-Phe-Arg-AMC.

Endotoxin

Less than 0.01 ng endotoxin/1μg hCystatin C.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hCystatin C. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

Cystatin C is a 14 kDa member of the Cystatin superfamily of cysteine protease inhibitors (1). Most cell types secrete Cystatin C (1). Cystatin C inhibits cathepsins, and thereby may function as a tumor suppressor by inhibiting cathepsin mediated tumor cell invasion (2). In addition, this tumor suppressor function can also be attributed to Cystatin C's ability to antagonize TGF-β1 signaling (2,3). Cystatin C may also modulate antigen presentation through its ability to inhibit cathepsins (4). Cystatin C is also a biomarker for kidney dysfunction (5).

  1. Keppler, D. (2006) Cancer Lett 235, 159-76.
  2. Sokol, J.P. and Schiemann, W.P. (2004) Mol Cancer Res 2, 183-95.
  3. Tian, M. and Schiemann, W.P. (2009) Transl Oncol 2, 174-83.
  4. Vray, B. et al. (2002) Cell Mol Life Sci 59, 1503-12.
  5. Madero, M. and Sarnak, M.J. (2009) Curr Opin Nephrol Hypertens 18, 258-63.

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