Cell Signaling Technology

Product Pathways - Protein Stability

SUMO-1 (2A12) Mouse mAb #5718

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous n/a Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SUMO-1 (2A12) Mouse mAb detects recombinant SUMO-1 and endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 90 kD). This antibody does not detect recombinant SUMO-2 or SUMO-3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a sequence within human SUMO-1 that does not correspond to SUMO-2/3.

Western Blotting

Western Blotting

Western blot analysis of recombinant GST-SUMO-1 protein, His-SUMO-2 and His-SUMO-3 using SUMO-1 (2A12) Mouse mAb (upper) and SUMO-2/3 (18H8) Rabbit mAb #4971 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from C6, Neuro-2a and CAD cells using SUMO-1 (2A12) Mouse mAb.

Background

Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11).

  1. Schwartz, D.C. and Hochstrasser, M. (2003) Trends Biochem. Sci. 28, 321-328.
  2. Kim, K.I. et al. (2002) J. Cell Physiol. 191, 257-268.
  3. Matunis, M.J. et al. (1996) J. Cell Biol. 135, 1457-1470.
  4. Duprez, E. et al. (1999) J. Cell Sci. 112, 381-393.
  5. Gostissa, M. et al. (1999) EMBO J. 18, 6462-6474.
  6. Rodriguez, M.S. et al. (1999) EMBO J. 18, 6455-6461.
  7. Desterro, J.M. et al. (1998) Mol. Cell 2, 233-239.
  8. Tatham, M.H. et al. (2001) J. Biol. Chem. 276, 35368-35374.
  9. Azuma, Y. et al. (2003) J. Cell Biol. 163, 477-487.
  10. Li, Y. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 259-264.
  11. Saitoh, H. and Hinchey, J. (2000) J. Biol. Chem. 275, 6252-6258.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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