Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Axl (Tyr702) (D12B2) Rabbit mAb #5724

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (M) (R) Endogenous 138 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Axl (Tyr702) (D12B2) Rabbit mAb detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases, such as EGFR.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr702 of human Axl protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H1299 cells, untreated or Gas6-treated (100 ng/ml for 10 minutes), using Phospho-Axl (Tyr702) (D12B2) Rabbit mAb (upper) or Axl (C44G1) Rabbit mAb #4566 (lower).

Background

Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

Phosphorylation of Axl Receptor on Tyr702 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines (6).

  1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
  2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
  3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
  4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.
  5. Rikova, K. et al. (2007) Cell 131, 1190-203.
  6. Guo, A. et al. (2008) Proc Natl Acad Sci USA 105, 692-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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