Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

ELP3 (D5H12) Rabbit mAb #5728

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R (Hm) (B) (Dg) (Pg) (Hr) Endogenous 62 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  B=Bovine  Dg=Dog  Pg=Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ELP3 (D5H12) Rabbit mAb recognizes endogenous levels of total ELP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly431 of human ELP3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ELP3 (D5H12) Rabbit mAb.

Background

Elongator is a highly conserved transcription elongation factor complex that was first identified in yeast as part of the hyperphosphorylated RNA polymerase II (RNAPII) holoenzyme (1). The Elongator complex consists of 6 subunits, ELP1-6, and has been shown to have acetyltransferase activity (2). The acetylation targets of Elongator include histone H3, which is linked to the transcription elongation function of the complex, and α-tubulin, which is associated with regulation of migration and maturation of projection neurons (3-6).

ELP3 is the catalytic acetyltransferase subunit of the Elongator complex (2,3). ELP3 contains an iron-sulfur cluster that can bind S-adenosylmethionine, which is necessary for the structural integrity of the Elongator and has been shown to play an important role in mediating global zygotic DNA demethylation of the paternal genome post-fertilization (7-9).

  1. Otero, G. et al. (1999) Mol Cell 3, 109-18.
  2. Creppe, C. and Buschbeck, M. (2011) J Biomed Biotechnol 2011, 924898.
  3. Wittschieben, B.O. et al. (1999) Mol Cell 4, 123-8.
  4. Hawkes, N.A. et al. (2002) J Biol Chem 277, 3047-52.
  5. Kim, J.H. et al. (2002) Proc Natl Acad Sci USA 99, 1241-6.
  6. Creppe, C. et al. (2009) Cell 136, 551-64.
  7. Paraskevopoulou, C. et al. (2006) Mol Microbiol 59, 795-806.
  8. Greenwood, C. et al. (2009) J Biol Chem 284, 141-9.
  9. Okada, Y. et al. (2010) Nature 463, 554-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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