Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Development

Notch2 (D76A6) XP® Rabbit mAb #5732

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M R Endogenous 110, 300 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

Notch2 (D76A6) XP® Rabbit mAb recognizes endogenous levels of total Notch2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2378 of human Notch2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and SK-MEL-5 cells using Notch2 (D76A6) XP® Rabbit mAb. The full-length (FL) Notch2 protein and the transmembrane/intracellular region (NTM) are indicated.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SW620 (red) and 293 (blue) cells using Notch2 (D76A6) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SNB19 cells using Notch2 (D76A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).

Notch2 is a member of the Notch family and mutation in Notch2 is associated with Alagille syndrome (5).

  1. Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
  2. Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
  3. Schroeter, E.H. et al. (1998) Nature 393, 382-6.
  4. Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
  5. McDaniell, R. et al. (2006) Am J Hum Genet 79, 169-73.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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