Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RalBP1 (D87H8) Rabbit mAb #5739

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 95 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RalBP1 (D87H7) Rabbit mAb detects endogenous levels of total RalBP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp623 of human RalBP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RalBP1 (D87H8) Rabbit mAb.

Background

The RalA binding protein 1 (RalBP1 or RLIP76) was originally identified as a GTP-RalA associated protein that acted as a downstream RalA effector in regulating Ral-Ras signaling (1). RalBP1 interacts with RalA and the endocytosis protein REPS2 (POB1) through its carboxy-terminal Ral binding domain. RalBP1 has an intrinsic GTPase activating function and interacts with Cdc42 through its centrally located Rho-GAP domain (1-3). A protein complex containing RalBP1/POB1/RalA regulates endocytosis of membrane receptors (4). RalBP1 also functions as a non-ABC transporter that catalyzes the ATP-dependent transport of numerous xenobiotics, including glutathione conjugates and some chemotherapeutic agents. RalBP1 transporter activity may play an important role in detoxification, drug resistance and the stress response (5-7). Increased expression of RalBP1 protein is associated with some forms of cancer and regression of cancer xenografts results from RalBP1 inhibition (8,9). Evidence to date suggests that RalBP1 may be a promising therapeutic target for cancer therapy.

  1. Cantor, S.B. et al. (1995) Mol Cell Biol 15, 4578-84.
  2. Matsubara, K. et al. (1997) FEBS Lett 410, 169-74.
  3. Ikeda, M. et al. (1998) J Biol Chem 273, 814-21.
  4. Nakashima, S. et al. (1999) EMBO J 18, 3629-42.
  5. Awasthi, Y.C. et al. (2007) Curr Drug Metab 8, 315-23.
  6. Awasthi, S. et al. (2002) Drug Metab Dispos 30, 1300-10.
  7. Awasthi, S. et al. (2008) Clin Cancer Res 14, 4372-7.
  8. Hudson, M.E. et al. (2007) Proc Natl Acad Sci USA 104, 17494-9.
  9. Singhal, S.S. et al. (2007) Cancer Res 67, 4382-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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