Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Arf6 (D12G6) Rabbit mAb #5740

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 19 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Arf6 (D12G6) Rabbit mAb detects endogenous levels of total Arf6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg100 of human Arf6 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Arf6 (D12G6) Rabbit mAb.

Background

ADP-ribosylation factor (Arf) proteins are low molecular weight GTP binding proteins that belong to the Ras GTPase superfamily (1). Arf proteins are grouped into three distinct classes based on amino acid sequence and structural similarity, with Arf6 as the single class III protein to date. Arf6 is localized mainly to the plasma membrane and endosomes (1,2). This small GTPase interacts with PIP5K, PLD and Rac1, proteins important in lipid metabolism and actin regulation. Arf6 function depends upon its cycling between GDP- and GTP-bound states, which is regulated by associated GAP and GEF factors (3,4). Plasma membrane-associated Arf6 appears to play several functions during the many steps of membrane trafficking, including regulating membrane receptor internalization in both clathrin-dependent and independent pathways, endosomal recycling, and proximal actin reorganization and remodeling (5,6).

  1. Gillingham, A.K. and Munro, S. (2007) Annu Rev Cell Dev Biol 23, 579-611.
  2. Donaldson, J.G. and Honda, A. (2005) Biochem Soc Trans 33, 639-42.
  3. Sakagami, H. (2008) Tohoku J Exp Med 214, 191-8.
  4. Casanova, J.E. (2007) Traffic 8, 1476-85.
  5. D'Souza-Schorey, C. and Chavrier, P. (2006) Nat Rev Mol Cell Biol 7, 347-58.
  6. Donaldson, J.G. (2003) J Biol Chem 278, 41573-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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