Product Pathways - Companion Products
Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| Endogenous | Rabbit IgG |
Applications Key:
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5742:
- Flow
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control (PE conjugate) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Source / Purification
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 (PI3 Kinase Inhibitor) #9901, Wortmannin (PI3 Kinase Inhibitor) #9551 and U0126 (MEK1/2 Inhibitor) #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (PE Conjugate) #5315 compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) (red).
Description
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
