Product Pathways - Companion Products
GST (91G1) Rabbit mAb (Biotinylated) #5746
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W | Transfected Only | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5746:
- Western Blotting
Specificity / Sensitivity
GST (91G1) Rabbit mAb (Biotinylated) detects transfected GST fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
Western Blotting
Western blot analysis of extracts from untransfected (-) and transfected cells overexpressing GST-Trcp1 (+), using GST (91G1) Rabbit mAb (Biotinylated).
Description
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as unconjugated GST (91G1) Rabbit mAb #2625.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
Application References
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Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
