Product Pathways - Tyrosine Kinase / Adaptors
EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated) #5747
|W||H||Endogenous||175 KDa||Rabbit IgG|
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (Biotinylated) detects endogenous levels of EGFR E746-A750del mutant protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb #2085.
The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.
- Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
- Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
- Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
- Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
- Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.
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For Research Use Only. Not For Use In Diagnostic Procedures.