Product Pathways - Companion Products
DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Sepharose Bead Conjugate) #5750
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IP | Transfected Only | Mouse IgG1 |
Applications Key:
IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5750:
- Immunoprecipitation
Specificity / Sensitivity
DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Sepharose Bead Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG® antibodies.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide.
IP
Immunoprecipitation of extracts from COS-7 cells, mock transfected (lanes 1, 3, and 5; -) or transfected with a construct expressing CBP/DDK-tagged full-length human Arrb1 (hArrb1-CBP/DDK; lanes 2, 4, and 6; +), using Mouse IgG (Sepharose Bead Conjugate) #3420 (lanes 1 and 2) or DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Sepharose Bead Conjugate) (lanes 3 and 4). Lanes 5 and 6 are 10% input. Western blot analysis was performed using DYKDDDDK Tag Antibody (HRP Conjugate) #2044.
Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (Sepharose Bead Conjugate) is useful for the immunoprecipitation of DYKDDDDK (Flag) tagged proteins.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
