Cell Signaling Technology

Product Pathways - Protein Stability

RCHY1 Antibody #5754

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M (Mk) Endogenous 29 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RCHY1 Antibody detects endogenous levels of total RCHY1 protein. This antibody does not detect RCHY1 dimers.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RCHY1 protein isoform1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RCHY1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a construct overexpressing murine RCHY1, using RCHY1 Antibody.

Background

RING finger and CHY1 zinc finger domain-containing protein 1 (RCHY1) is a newly identified RING-H2-type protein-ubiquitin E3 ligase that is expressed as multiple isoforms generated through alternative splicing of mRNA transcripts (1,2). Notably, RCHY1 is a p53-regulated gene and multiple studies have shown that RCHY1 directly binds p53 to promote its ubiquitin-dependent proteasomal degradation, ultimately leading to repression of p53 growth suppressive transcriptional activity (1,3). RCHY1 is also likely to promote deregulated cell proliferation by targeting the CDK inhibitor, p27 Kip1, for proteasomal degradation (4). In agreement with its growth promoting activity, RCHY1 overexpression has been linked to cancer of the lung (5,6) and prostate (7).

  1. Corcoran, C.A. et al. (2009) J Biol Chem 284, 21955-70.
  2. Shi, J. et al. (2010) Mol Cell Pharmacol 2, 21-23.
  3. Leng, R.P. et al. (2003) Cell 112, 779-91.
  4. Hattori, T. et al. (2007) Cancer Res 67, 10789-95.
  5. Duan, W. et al. (2004) J Natl Cancer Inst 96, 1718-21.
  6. Duan, W. et al. (2006) Exp Cell Res 312, 3370-8.
  7. Logan, I.R. et al. (2006) Mol Cell Biol 26, 6502-10.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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