Product Pathways - Translational Control
GβL (86B8) Rabbit mAb (Sepharose Bead Conjugate) #5837
PhosphoSitePlus® protein, site, and accession data: LST8
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IP | H M R Mk | Endogenous | 37 | Rabbit IgG |
Applications Key:
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5837:
- Immunoprecipitation
Specificity / Sensitivity
GβL (86B8) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total GβL protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln210 of human GβL protein.
IP
Immunoprecipitation of extracts from 293 cells using GβL (86B8) Rabbit mAb (Sepharose Bead Conjugate) (lane 1). Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 was used as a negative control (lane 2). The western blot was probed using GβL (86B8) Rabbit mAb #3274 and detected with Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb #3678 followed by Anti-mouse IgG, HRP-linked Antibody #7076.
Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. GβL (86B8) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of GβL protein.
Background
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).
- Sarbassov, D.D. et al. (2004) Curr Biol. 14, 1296-1302.
- Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
Application References
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Companion Products
- 3423 Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate)
- 3274 GβL (86B8) Rabbit mAb
- 3678 Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
