Cell Signaling Technology

Product Pathways - Metabolism

SDHA Antibody #5839

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 70 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SDHA Antibody recognizes endogenous levels of total SDHA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SDHA protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SDHA Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using SDHA Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Background

Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington’s disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas (4,5).

  1. Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
  2. Bourgeron, T. et al. (1995) Nat Genet 11, 144-9.
  3. Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63.
  4. Baysal, B.E. et al. (2000) Science 287, 848-51.
  5. Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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