Product Pathways - Neuroscience
GAD2 (D5G2) XP® Rabbit mAb #5843
PhosphoSitePlus® protein, site, and accession data: GAD2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F | H M R | Endogenous | 60 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
GAD2 (D5G2) XP® Rabbit mAb recognizes endogenous levels of total GAD2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GAD2 protein.
Western Blotting
Western blot analysis of extracts from neonatal mouse and fetal rat brain using GAD2 (D5G2) XP® Rabbit mAb.
IF-F
Confocal immunofluorescent analysis of mouse brain using GAD2 (D5G2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA marker).
Background
The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6).
- Kaufman, D.L. et al. (1991) J Neurochem 56, 720-3.
- Feldblum, S. et al. (1993) J Neurosci Res 34, 689-706.
- Gao, B. and Moore, R.Y. (1996) J Biol Rhythms 11, 172-9.
- Rubi, B. et al. (2001) J Biol Chem 276, 36391-6.
- Gilliam, L.K. et al. (2004) Clin Exp Immunol 138, 337-41.
- Skorstad, G. et al. (2008) Eur J Neurol 15, 973-80.
Application References
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Companion Products
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 5305 GAD1 Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
