Cell Signaling Technology

Product Pathways - Translational Control

RMP Antibody #5844

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 79 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RMP Antibody recognizes endogenous levels of total RMP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RMP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RMP Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, either mock transfected (-) or transfected with human RMP encoding transcript variant 1 (+), using RMP Antibody.

Background

RMP (RPB5-Mediating Protein), also known as URI (Unconventional prefoldin RBP5 Interactor), was described as an unconventional member of the prefoldin (PFD) family of chaperones that are involved in actin and tubulin folding (1-4). Like conventional members of the α-class of PFDs, RMP contains N- and C-terminal α-helical coiled-coil structures connected by two β hairpins. In addition, RMP possesses an RPB5-binding segment and a long C-terminal acidic segment. It is posited that RMP exists as a component of a macromolecular complex within human cells and functions as a molecular scaffold to assemble a PFD complex containing other PFDs and proteins with functions in transcription and ubiquitination. Indeed, evidence is provided that RMP negatively modulates RNA polymerase II-dependent transcription by binding to TFIIF (5) and RBP5 (6) and is involved in mTOR signaling by coordinating the regulation of nutrient availability with gene expression (1). In accord with its ability to coordinate gene expression with nutrient availability, RMP was shown to be a mitochondrial substrate of S6K1. S6K1-mediated phosphorylation of RMP at Ser371 triggers a series of biochemical events that constitute a negative feedback loop, in part, aimed at restraining S6K1 survival signaling and ensuring that the mitochondrial threshold for apoptosis corresponds to availability of nutrients and growth factors (7).

  1. Gstaiger, M. et al. (2003) Science 302, 1208-12.
  2. Vainberg, I.E. et al. (1998) Cell 93, 863-73.
  3. Martín-Benito, J. et al. (2002) EMBO J 21, 6377-86.
  4. Geissler, S. et al. (1998) EMBO J 17, 952-66.
  5. Wei, W. et al. (2003) Cell Res 13, 111-20.
  6. Dorjsuren, D. et al. (1998) Mol Cell Biol 18, 7546-55.
  7. Djouder, N. et al. (2007) Mol Cell 28, 28-40.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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