Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

NDRG3 Antibody #5846

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 45 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NDRG3 Antibody recognizes endogenous levels of total NDRG3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NDRG3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using NDRG3 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a mouse NDRG3 construct (+), using NDRG3 Antibody.

Background

The NDRG (N-Myc downstream-regulated gene) family, consisting of NDRG1, NDRG2, NDRG3, and NDRG4, are a group of structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was up-regulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues, and have been suggested to function as a tumor suppressors (4,5).

Members of the NDRG family each have distinct expression patterns; NDRG3 is most highly expressed in the testis, prostate, ovary, and brain (2,6,7). NDRG3 has also been shown to be up-regulated by androgen in prostate cell lines and can promote cell growth in those cells (8).

  1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
  2. Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
  3. Zhou, R.H. et al. (2001) Genomics 73, 86-97.
  4. Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
  5. Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
  6. Okuda, T. and Kondoh, H. (1999) Biochem Biophys Res Commun 266, 208-15.
  7. Zhao, W. et al. (2001) Biochim Biophys Acta 1519, 134-8.
  8. Wang, W. et al. (2009) Int J Cancer 124, 521-30.

Application References

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