Cell Signaling Technology

Product Pathways - DNA Damage

RecQL5 (1A2) Mouse mAb #5847

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 120 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RecQL5 (1A2) Mouse mAb recognizes endogenous levels of total RecQL5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human RecQL5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Daudi and 293 cells using RecQL5 (1A2) Mouse mAb.

Background

The RecQ family is a group of DNA helicases that play an important role in global genomic stability (1). Mutations in three of the five known human RecQ proteins (BLM, WRN, and RECQL4) give rise to clinically distinct disorders that are characterized by features such as premature aging and predisposition to cancer (2,3). The clinical distinction of each disease associated with these mutations points to distinct roles that members of this helicase family play in DNA metabolism. The RecQL5 helicase has not yet been associated with any human disease, but RecQL5 -/- mice exhibit an increased incidence of cancer (4,5). It has recently been shown that RecQL5 protects genome stability through two parallel mechanims: helicase action and interaction with the initiation form of RNA Polymerase II (6). It has also been shown that RecQL5 -/- mouse embryonic stem cells display an elevated frequency of sister chromatic exchange (SCE), suggesting a role in suppression of homologous recombination and/or crossover events (7,8).

  1. Chu, W.K. and Hickson, I.D. (2009) Nat Rev Cancer 9, 644-54.
  2. Hanada, K. and Hickson, I.D. (2007) Cell Mol Life Sci 64, 2306-22.
  3. Dietschy, T. et al. (2007) Cell Mol Life Sci 64, 796-802.
  4. Hu, Y. et al. (2007) Genes Dev 21, 3073-84.
  5. Bachrati, C.Z. and Hickson, I.D. (2008) Chromosoma 117, 219-33.
  6. Islam, M.N. et al. (2010) Mol Cell Biol 30, 2460-72.
  7. Hu, Y. et al. (2005) Mol Cell Biol 25, 3431-42.
  8. Hu, Y. et al. (2009) Mol Biol Cell 20, 114-23.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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