Product Pathways - Stem Cell and Lineage Markers
GATA-6 (D61E4) XP® Rabbit mAb #5851
|W IF-IC||H (M) (R) (Dg) (Pg)||Endogenous||55||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
GATA-6 (D61E4) XP® Rabbit mAb recognizes endogenous levels of total GATA-6 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-6 protein.
Western blot analysis of extracts from Huh7 and 293 cells using GATA-6 (D61E4) XP® Rabbit mAb.
GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).
GATA-6 plays a critical role in endoderm development (4). It is essential for development of the heart, gut, and other organs (5,6). Knock out of GATA-6 is embryonic lethal due to defects in formation of the heart tube and a failure to develop extraembryonic endoderm (4). Loss of expression, or loss of nuclear localization of GATA-6 is apparent in a large number of ovarian tumors (7).
- Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
- Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
- Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
- Cai, K.Q. et al. (2008) Dev Dyn 237, 2820-9.
- Charron, F. and Nemer, M. (1999) Semin Cell Dev Biol 10, 85-91.
- Haveri, H. et al. (2008) BMC Gastroenterol 8, 9.
- Caslini, C. et al. (2006) Oncogene 25, 5446-61.
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For Research Use Only. Not For Use In Diagnostic Procedures.