Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Stem Cell and Lineage Markers

GATA-3 (D13C9) XP® Rabbit mAb #5852

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC F H (Mk) Endogenous 48 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GATA-3 (D13C9) XP® Rabbit mAb recognizes endogenous levels of total GATA-3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GATA-3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from CEM and SH-SY5Y cells using GATA-3 (D13C9) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GATA-3 (D13C9) XP® Rabbit mAb (left) or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, MCF-7 (left) or HUVEC (right), using GATA-3 (D13C9) XP® Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells (blue) and MCF7 cells (green) using GATA-3 (D13C9) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal Immunofluorescent analysis of MCF7 (left) and HUVE (right) cells using GATA-3 (D13C9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).

GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 null mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12).

  1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
  2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
  3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
  4. Debacker, C. et al. (1999) Mech Dev 85, 183-7.
  5. Pandolfi, P.P. et al. (1995) Nat Genet 11, 40-4.
  6. Ho, I.C. et al. (2009) Nat Rev Immunol 9, 125-35.
  7. Amsen, D. et al. (2007) Immunity 27, 89-99.
  8. Grote, D. et al. (2008) PLoS Genet 4, e1000316.
  9. Kaufman, C.K. et al. (2003) Genes Dev 17, 2108-22.
  10. Kouros-Mehr, H. et al. (2006) Cell 127, 1041-55.
  11. Chou, J. et al. (2010) J Cell Physiol 222, 42-9.
  12. Van Esch, H. et al. (2000) Nature 406, 419-22.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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