Product Pathways - Development
WTX (D38E5) Rabbit mAb #5854
PhosphoSitePlus® protein, site, and accession data: WTX
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Transfected Only | 200 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5854:
- Western Blotting
Specificity / Sensitivity
WTX (D38E5) Rabbit mAb detects transfected levels of total WTX protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human WTX protein.
Western Blotting
Western blot analysis of extracts from HeLa cells, untransfected or transfected with a contruct overexpressing human WTX, using WTX (D38E5) Rabbit mAb.
Background
WTX (Wilms’ tumor gene on the X chromosome) is a developmentally regulated gene with a potentially important role in kidney development (1). Functional studies of WTX suggest that it acts as a tumor suppressor gene in renal cells by promoting β-catenin ubiquitination and degradation, thereby antagonizing WNT/β-catenin signaling (1,2). WTX is found to be inactivated in 30% of Wilms’ tumors, mostly by chromosomal deletion (3). Wilms’ tumor is a pediatric kidney cancer that arises from cells that fail to differentiate during kidney development (4). Inactivation of the WT1 tumor suppressor gene accounts for 10-15% of Wilms’ tumor cases (5). WTX has been shown to enhance WT1-mediated transactivation, suggesting a physiologically significant interaction between WT1 and WTX (6). WTX may be directly involved in the transcriptional regulation of cellular differentiation in the kidney through interactions with WT1 and other transcription factors (6).
- Rivera, M.N. et al. (2007) Science 315, 642-5.
- Major, M.B. et al. (2007) Science 316, 1043-6.
- Kim, M.S. et al. (2010) J Biol Chem 285, 14585-93.
- Dome JS, Huff V. (2003) GeneReviews
- Hohenstein, P. and Hastie, N.D. (2006) Hum Mol Genet 15 Spec No 2, R196-201.
- Rivera, M.N. et al. (2007) Science 315, 642-5.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
