Product Pathways - Lymphocyte Signaling
NFAT1 (D43B1) XP® Rabbit mAb #5861
|5861S||100 µl (10 western blots)||---||In Stock||---|
|5861P||40 µl (4 western blots)||---||In Stock||---|
|5861||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||140||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
NFAT1 (D43B1) XP® Rabbit mAb detects endogenous levels of total NFAT1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly87 of human NFAT1 protein.
Western blot analysis of extracts from Ramos, Raji, and EL4 cells using NFAT1 (D43B1) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using NFAT1 (D43B1) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using NFAT1 (D43B1) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using NFAT1 (D43B1) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded cell pellets, Jurkat (left) or LNCaP (right), using NFAT1 (D43B1) XP® Rabbit mAb.
Flow cytometric analysis of HCT 116 using NFAT1 (D43B1) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
The NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response (1,2). NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells (3). In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus (2). Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1 (3,4). Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.