Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Metabolism

CA12 (D75C6) XP® Rabbit mAb #5864

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP F H Endogenous 45-50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

CA12 (D75C6) XP® Rabbit mAb detects endogenous levels of total CA12 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly39 of human CA12 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-OV-3 and MCF7 cells using CA12 (D75C6) XP® Rabbit mAb.

Flow Titer

Flow Titer

Flow cytometric analysis of A-204 cells (blue) and MCF7 cells (green) using CA12 (D75C6) XP® Rabbit mAb.

Background

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2).

CA12, a member of the α class, is a plasma membrane protein with the catalytic domain in the extracellular space. CA12 is widely expressed in many tissues and is found at high levels in renal cell cancers. Its expression is induced by hypoxia in solid tumors and is considered to be a tumor marker (3-5). Genetic mutations in CA12 are associated with hyperchlorhidrosis (6,7).

  1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
  2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.
  3. Chiche, J. et al. (2009) Cancer Res 69, 358-68.
  4. Barnett, D.H. et al. (2008) Cancer Res 68, 3505-15.
  5. Watson, P.H. et al. (2003) Br J Cancer 88, 1065-70.
  6. Feldshtein, M. et al. (2010) Am J Hum Genet 87, 713-20.
  7. Muhammad, E. et al. (2011) Hum Genet 129, 397-405.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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