Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

TIF1β (4E1) Mouse mAb #5868

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H Endogenous 100, 75 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TIF1β (4E1) Mouse mAb recognizes endogenous levels of total TIF1β protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TIF1β protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TIF1β (4E1) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using TIF1β (4E1) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Background

TIF1β is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1β is a corepressor for KRAB (Kruppel associated box) domain containing zinc finger proteins. The KRAB domain containing zinc finger proteins are a large group of transcription factors that are vertebrate-specific, varied in their expression patterns between species, and thought to regulate gene transcription programs that control speciation (3,4).TIF1β has been shown to be essential for early embryonic development and spermatogenesis (6,5). It functions to either activate or repress transcription in response to environmental or developmental signals by chromatin remodeling and histone modification. The recruitment and association of TIF1β with heterochromatin protein (HP1) is essential for transcriptional repression, and for progression through differentiation of F9 embryonic carcinoma cells (6,7). TIF1β also plays a role in the DNA damage response. Phosphorylation of TIF1β on Ser842 occurs in an ATM-dependent manner in response to genotoxic stress and is thought to be essential for chromatin relaxation, which is in turn required for the DNA damage response (8).

  1. Le Douarin, B. et al. (1995) EMBO J. 14, 2020-2033.
  2. Le Douarin, B. et al. (1996) EMBO J. 15, 6701-6715.
  3. Friedman, J.R. et al. (1996) Genes Dev. 10, 2067-2078.
  4. Krebs, C.J. et al. (2005) Genomics 85, 752-761.
  5. Weber, P. et al. (2002) Development 129, 2329-2337.
  6. Cammas, F. et al. (2004) Genes Dev. 18, 2147-2160.
  7. Cammas, F. et al. (2007) Differentiation 75, 627-37.
  8. Ziv, Y. et al. (2006) Nat. Cell Biol. 8, 870-876.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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