Product Pathways - Companion Products
Mouse IgG (Magnetic Bead Conjugate) #5873
| Applications | Reactivity | Source |
|---|---|---|
| IP | Mouse |
Applications Key:
IP=Immunoprecipitation
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5873:
- Immunoprecipitation
Specificity / Sensitivity
Normal mouse IgG is not expected to recognize any specific antigen.
Source / Purification
Normal mouse IgG was isolated by affinity chromatography from naive mouse serum.
IP
Immunoprecipitation using NIH/3T3 (lane 1 and 2) and C6 (lane 3 and 4) cell extracts using Mouse IgG (Magnetic Bead Conjugate) (lane 1 and 3) or Akt (pan) (40D4) Mouse mAb (Magnetic Bead Conjugate) #5697 (lane 2 and 4). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.
Description
This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic beads. Mouse IgG (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Directions for Use
Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.
For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.
Background
Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
