Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

GRK6 (D1A4) Rabbit mAb #5878

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Endogenous 70 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GRK6 (D1A4) Rabbit mAb recognizes endogenous levels of total GRK6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu89 of human GRK6 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GRK6 (D1A4) Rabbit mAb.

Background

G protein-coupled Receptor Kinase 6 (GRK6) is one of 7 members of the GRK serine/threonine kinase subfamily, which are known primarily for their role in desensitizing activated G protein-coupled receptors (GPCRs) (1,2). GRKs function by phosphorylating serine/threonine residues in activated GPCRs. Upon phosphorylation these residues serve as binding sites for β-arrestin proteins, inhibiting re-activation of GPCRs by blocking their re-association with G proteins (3). There is evidence that GRKs can also modulate selected non-GPCR signaling pathways (2). For example, GRK6 has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (4), and the insulin-like growth factor signaling pathway (5). GRK6 may also play a role in immune system function. Investigators have found GRK6 expression is typically abundant in hematopoietic tumor cell lines, and a recent research study demonstrated that GRK6 suppression was selectively lethal for a number of myeloma tumor cell lines (6).

  1. Mushegian, A. et al. (2012) PLoS One 7, e33806.
  2. Gurevich, E.V. et al. (2012) Pharmacol Ther 133, 40-69.
  3. Ribas, C. et al. (2007) Biochim Biophys Acta 1768, 913-22.
  4. Chen, M. et al. (2009) J Biol Chem 284, 35040-8.
  5. Zheng, H. et al. (2012) Proc Natl Acad Sci U S A 109, 7055-60.
  6. Tiedemann, R.E. et al. (2010) Blood 115, 1594-604.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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