Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

eNOS (6H2) Mouse mAb #5880

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H B Endogenous 140 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eNOS (6H2) Mouse mAb recognizes endogenous levels of total eNos protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human eNOS protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from BAEC and HUVEC cells using eNOS (6H2) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using eNOS (6H2) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using eNOS (6H2) Mouse mAb.


Background

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

  1. Fulton, D. et al. (2001) J. Pharmacol. Exp. Ther. 299, 818-824.
  2. Shaul, P.W. (2002) Annu. Rev. Physiol. 64, 749-774.
  3. Chen, Z.P. et al. (1999) FEBS Lett. 443, 285-289.
  4. Dimmeler, S. et al. (1999) Nature 399, 601-605.
  5. Fulton, D. et al. (1999) Nature 399, 597-601.
  6. Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
  7. Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products