Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Desmoplakin (Ser165/166) Antibody #5885

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 250 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Desmoplakin (Ser165/166) Antibody recognizes endogenous levels of desmoplakin protein only when phosphorylated at Ser165/166.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser165/166 of human desmoplakin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 and Calu-3 cells, untreated or treated with calf intestinal phosphatase (CIP) and λ phosphatase, using Phospho-Desmoplakin (Ser165/166) Antibody.

Background

Desmosomes are a class of intracellular junction that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein Desmoplakin, which acts as a tether by binding the cytoplasmic component of the Desmosome at it’s N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8).

Phospho-Desmoplakin (Ser165/166) Antibody is directed against a site that was identified at Cell Signaling Technology (CST) using PTMScan®, our LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus®, a modification site knowledgebase developed and maintained by CST, at www.phosphosite.org for more information.

  1. Stokes, D.L. (2007) Curr Opin Cell Biol 19, 565-71.
  2. Kowalczyk, A.P. et al. (1997) J Cell Biol 139, 773-84.
  3. Meng, J.J. et al. (1997) J Biol Chem 272, 21495-503.
  4. Green, K.J. and Simpson, C.L. (2007) J Invest Dermatol 127, 2499-515.
  5. Vasioukhin, V. et al. (2001) Nat Cell Biol 3, 1076-85.
  6. Rampazzo, A. et al. (2002) Am J Hum Genet 71, 1200-6.
  7. Armstrong, D.K. et al. (1999) Hum Mol Genet 8, 143-8.
  8. Jonkman, M.F. et al. (2005) Am J Hum Genet 77, 653-60.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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