Product Pathways - Neuroscience
γ Secretase Antibody Sampler Kit #5887
|5887S||1 Kit (4 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Nicastrin (D38F9) Rabbit mAb #5665||40 µl||W||H, M, R, Hm, Mk||110, 120||Rabbit IgG|
|PEN2 Antibody #5451||40 µl||W||H, M, R, Mk||13||Rabbit|
|Presenilin 1 (D39D1) Rabbit mAb #5643||40 µl||W, IP||H, M, R, Mk||22 (CTF)||Rabbit IgG|
|Presenilin 2 Antibody #2192||40 µl||W, IP||H, M, R, Mk||23 (C-terminal fragment). 54 (full-length).||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey
Western blot analysis of extracts from human (293 and HeLa), mouse (NIH/3T3 and L929) and rat (C6 and PC12) cell lines using Presenilin 2 Antibody #2192.
Western blot analysis of extracts from various cell lines using PEN2 Antibody #5451.
Western blot analysis of extracts from HeLa, wild-type MEF, and Presenilin 1(-/-) MEF cells using Presenilin 1 (D39D1) Rabbit mAb #5643 (upper) and α/β-Tubulin Antibody #2148 (lower). (Wild-type MEF and Presenilin 1(-/-) MEF cells were kindly provided by Dr. Bart De Strooper, K.U.Leuven, Belgium).
The γ Secretase Antibody Sampler Kit provides an economical means of evaluating components of the gamma secretase complex. The kit contains enough primary and secondary antibodies to perform four western miniblot experiments.
Specificity / Sensitivity
Presenilin 1 (D39D1) Rabbit mAb recognizes endogenous levels of total Presenilin 1 protein. The antibody may detect a nonspecific band at 60 kDa. Presenilin 2 Antibody detects endogenous levels of the carboxy-terminal fragment of Presenilin 2 (23 kDa) and the full length protein (54 kDa) to a lesser extent. It does not cross-react with endogenous levels of presenilin 1. Nicastrin (D38F9) Rabbit mAb recognizes endogenous levels of total Nicastrin protein. PEN2 Antibody detects endogenous levels of total PEN2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with: a synthetic peptide corresponding to residues surrounding Ser330 of human Presenilin 2 or a synthetic peptide corresponding to residues surrounding Lys11 of human PEN2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with: a recombinant protein specific to the carboxy terminus of human Presenilin 1 protein or a synthetic peptide corresponding to residues surrounding Val390 of human Nicastrin protein.
The γ secretase protease complex interacts with and cleaves intramembrane substrates as an essential function for regulation of intracellular signaling and cell-cell interactions. This multiprotein complex is comprised of four integral membrane proteins, Presenilin, Nicastrin, Aph-1, and PEN2, all of which are essential for complete proteolytic activity (1). Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin’s associated γ-secretase activity for amyloid-β protein processing (2,3). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (3,4). Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (5,6). Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (7). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to γ-secretase to ensure the correct cleavage process (6,8). Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (9). PEN2 is an important part of the γ-secretase complex as its knock down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (10).
- Hansson, C.A. et al. (2004) J Biol Chem 279, 51654-60.
- Haass, C. and De Strooper, B. (1999) Science 286, 916-9.
- Kimberly, W.T. et al. (2000) J Biol Chem 275, 3173-8.
- Kim, T.W. et al. (1997) J Biol Chem 272, 11006-10.
- Yu, G. et al. (2000) Nature 407, 48-54.
- Esler, W.P. et al. (2002) Proc Natl Acad Sci U S A 99, 2720-5.
- Kopan, R. and Goate, A. (2002) Neuron 33, 321-4.
- Chen, F. et al. (2001) Nat Cell Biol 3, 751-4.
- Sala Frigerio, C. et al. (2005) J Neurol 252, 1033-6.
- Steiner, H. et al. (2002) J Biol Chem 277, 39062-5.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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