Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

LATS2 (D83D6) Rabbit mAb #5888

Applications Reactivity Sensitivity MW (kDa) Isotype
W H (Mk) Transfected Only 150 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LATS2 (D83D6) Rabbit mAb detects transfected levels of total LATS2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LATS2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected (-) or transfected with human LATS2 (+), using LATS2 (D83D6) Rabbit mAb.

Background

LATS2 is an AGC kinase of the NDR family of kinases. It is a tumor suppressor of the LATS family, and plays a critical role in centrosome duplication, maintenance of mitotic fidelity, and genomic stability (1). LATS2 inhibits cell growth at the G1/S transition by down-regulating cyclin E/CDK2 kinase activity (2). It also plays an important role in regulation of the Hippo pathway, a pathway that is important in controlling organ size and tumor progression by regulating cellular proliferation and promoting apoptosis (3,4,5)

  1. Yabuta, N. et al. (2007) J Biol Chem 282, 19259-71.
  2. Li, Y. et al. (2003) Oncogene 22, 4398-405.
  3. Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
  4. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
  5. Visser, S. and Yang, X. (2010) Cell Cycle 9, 3892-903.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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