Product Pathways - Translational Control
DHX29 (D19E10) Rabbit mAb #5926
PhosphoSitePlus® protein, site, and accession data: DDX29
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M Mk | Endogenous | 155 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
DHX29 (D19E10) Rabbit mAb recognizes endogenous levels of total DHX29 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DHX29 protein.
Western Blotting
Western blot analysis of extracts from HT-29 and HeLa cells using DHX29 (D19E10) Rabbit mAb.
Background
DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5' untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).
- de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8.
- Pisareva, V.P. et al. (2008) Cell 135, 1237-50.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
