Cell Signaling Technology

Product Pathways - Metabolism

TBC1D1 (G689) Antibody #5929

Applications Reactivity Sensitivity MW (kDa) Source
W IP M (H) Endogenous 160 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TBC1D1 (G689) Antibody detects endogenous levels of total TBC1D1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Gly 689 of human TBC1D1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 myotubes using TBC1D1 (G689) Antibody.

Background

TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5).

  1. Taylor, E.B. et al. (2008) J Biol Chem 283, 9787-96.
  2. Roach, W.G. et al. (2007) Biochem J 403, 353-8.
  3. Stone, S. et al. (2006) Hum Mol Genet 15, 2709-20.
  4. Chavez, J.A. et al. (2008) J Biol Chem 283, 9187-95.
  5. Vichaiwong, K. et al. (2010) Biochem J 431, 311-20.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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