Cell Signaling Technology

Product Pathways - Lineage Markers

LIN28A (6D1F9) Mouse mAb #5930

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H Endogenous 26 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LIN28A (6D1F9) Mouse mAb detects endogenous levels of total LIN28A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human LIN28A recombinant protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LIN28 (6D1F9) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using LIN28A (6D1F9) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as ras, myc, and Hmga2 (3). It has recently been shown that LIN28A and LIN28B are upregulated in primary human tumors and in human cancer cell lines, their overexpression directly correlating to downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5) and allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

  1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
  2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
  3. Cho, W.C. (2007) Mol Cancer 6, 60.
  4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
  5. West, J.A. et al. (2009) Nature 460, 909-13.
  6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
  7. Yu, J. et al. (2007) Science 318, 1917-20.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products