Cell Signaling Technology

Product Pathways - Development

SP1 Antibody #5931

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 90 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SP1 Antibody detects endogenous levels of total SP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro593 of human SP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from RL7, SR, and Ramos cells using SP1 Antibody.

Background

Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, EGR1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors, such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitination, and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). Investigators have found high levels of SP1 in patients with Alzheimer's disease (7).

  1. Kadonaga, J.T. et al. (1987) Cell 51, 1079-90.
  2. Song, J. et al. (2003) Int J Mol Med 11, 547-53.
  3. Tan, N.Y. and Khachigian, L.M. (2009) Mol Cell Biol 29, 2483-8.
  4. Majumdar, G. et al. (2003) Am J Physiol Endocrinol Metab 285, E584-91.
  5. Majumdar, G. et al. (2006) J Biol Chem 281, 3642-50.
  6. Solomon, S.S. et al. (2008) Life Sci 83, 305-12.
  7. Citron, B.A. et al. (2008) J Neurosci Res 86, 2499-504.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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