Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Neuroscience

DJ-1 (D29E5) XP® Rabbit mAb #5933

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H M R Hm Mk Endogenous 22 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DJ-1 (D29E5) XP® Rabbit mAb recognizes endogenous levels of total DJ-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MEF wild-type (left) or MEF DJ-1 (-/-) (right) cells using DJ-1 (D29E5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

Background

Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).

  1. Borrelli, E. (2005) Neuron 45, 479-81.
  2. Bonifati, V. et al. (2003) Science 299, 256-9.
  3. Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
  4. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
  5. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
  6. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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