Cell Signaling Technology

Product Pathways - Metabolism

VMS1 (D10E6) Rabbit mAb #5937

Applications Reactivity Sensitivity MW (kDa) Isotype
W M Endogenous 100 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VMS1 (D10E6) Rabbit mAb detects endogenous levels of total VMS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly332 of mouse VMS1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using VMS1 (D10E6) Rabbit mAb.

Background

Mitochondria are membrane-bound subcellular organelles critical to many functions in eukaryotic cells (1). Several mechanisms have been found to play a role in mitochondria quality control (2). A recent study identified mitochondria-associated protein degradation as one such mechanism (1,2). The study showed that VMS1 (VCP/Cdc48-associated mitochondrial stress-responsive) interacts with VCP/Cdc48 and Npl4, two components in the endoplasmic reticulum-associated protein degradation pathway, and mediates the degradation of damaged mitochondrial proteins under stress conditions (1). Defective VMS1 compromises mitochondrial functions and cell viability (1).

  1. Heo, J.M. et al. (2010) Mol Cell 40, 465-80.
  2. Chatenay-Lapointe, M. and Shadel, G.S. (2010) Cell Metab 12, 559-60.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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