Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Apoptosis

AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate) #5939

Applications Reactivity Sensitivity MW (kDa) Isotype
IP H M R Mk (B) (Dg) Endogenous 67 Rabbit IgG

Applications Key:  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate) recognizes endogenous levels of total AIF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to redidues surrounding Ala520 of human AIF protein.

IP

IP

Immunoprecipitation of Jurkat, NIH/3T3, and HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 and AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate). The western blot was probed using AIF (D39D2) XP® Rabbit mAb #5318 and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. AIF (D39D2) XP® Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of AIF. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated AIF (D39D2) XP® Rabbit mAb #5318.

Background

Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7).

  1. Daugas, E. et al. (2000) FEBS Lett 476, 118-23.
  2. Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111, 147-50.
  3. Susin, S.A. et al. (1999) Nature 397, 441-6.
  4. Joza, N. et al. (2001) Nature 410, 549-54.
  5. Ye, H. et al. (2002) Nat Struct Biol 9, 680-4.
  6. Miramar, M.D. et al. (2001) J Biol Chem 276, 16391-8.
  7. Klein, J.A. et al. (2002) Nature 419, 367-74.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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