Product Pathways - Translational Control
Mili (D14F5) XP® Rabbit mAb #5940
PhosphoSitePlus® protein, site, and accession data: PIWIL2
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-F | M | Endogenous | 110 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Mili (D14F5) XP® Rabbit mAb recognizes endogenous levels of total Mili protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Leu118 of mouse Mili protein.
Western Blotting
Western blot analysis of extracts from mouse testis and mouse brain using Mili (D14F5) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse testis using Mili (D14F5) XP® Rabbit mAb.
IF-F
Confocal immunofluorescent analysis of mouse testis using Mili (D14F5) XP® Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The Drosophila piwi gene was identified as being required for the self-renewal of germ-line stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and human (1). Miwi and Mili proteins are both mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mouse (3-5).
- Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
- Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
- Aravin, A. et al. (2006) Nature 442, 203-7.
- Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
- Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
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For Research Use Only. Not For Use In Diagnostic Procedures.
