Product Pathways - Cytoskeletal Signaling
COX IV (3E11) Rabbit mAb (Biotinylated) #6014
|W||H R Mk Z B Pg||Endogenous||17||Rabbit IgG|
Reactivity Key: H=Human R=Rat Mk=Monkey Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb (Biotinylated) detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein.
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated COX IV (3E11) Rabbit mAb #4850.
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3999 Streptavidin-HRP
- 4850 COX IV (3E11) Rabbit mAb
- 4844 COX IV Antibody
- 5247 COX IV (3E11) Rabbit mAb (HRP Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.