Product Pathways - Cytoskeletal Signaling
β-Tubulin (9F3) Rabbit mAb (Biotinylated) #6181
PhosphoSitePlus® protein, site, and accession data: TUBB
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk Z B | Endogenous | 55 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Z=Zebrafish
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 6181:
- Western Blotting
Specificity / Sensitivity
β-Tubulin (9F3) Rabbit mAb (Biotinylated) detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-tubulin.
Western Blotting
Western blot analysis of extracts from NIH/3T3, HeLa and PC-12 cells, using β-Tubulin (9F3) Rabbit mAb (Biotinylated) and detected using Streptavidin-HRP #3999.
Description
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Tubulin (9F3) Rabbit mAb #2128.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3999 Streptavidin-HRP
- 2128 β-Tubulin (9F3) Rabbit mAb
- 2146 β-Tubulin Antibody
- 2144 α-Tubulin Antibody
- 2125 α-Tubulin (11H10) Rabbit mAb
- 2148 α/β-Tubulin Antibody
- 4285 α-Tubulin (11H10) Rabbit mAb (Biotinylated)
- 9099 α-Tubulin (11H10) Rabbit mAb (HRP Conjugate)
- 5346 β-Tubulin (9F3) Rabbit mAb (HRP Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
