Product Pathways - siRNA
SignalSilence® Control siRNA (Fluorescein Conjugate) #6201
Western Blotting
Western blot analysis to assess NF-kappaB p65 expression 48 hours post-transfection with SignalSilence NF-kappaB p65 siRNA #6261 or with SignalSilence® Control siRNA at various concentrations. Transfection reagent is supplied by Mirus.
Fluorescent Detection
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) in living Hela cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. Transfection reagent is supplied by Mirus.
Directions for Use
Transfection protocol and recommended cell lines will vary with the transfection reagent used. Please refer to transfection reagent manufacturer's protocol.
Background
RNA interference is a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. SignalSilence TM Control siRNA (Fluorescein Conjugate) is an siRNA sequence that will not lead to the specific degradation of any cellular message. It is intended to serve as a negative control for experiments using targeted siRNA transfection. In addition, this siRNA is fluorescein-conjugated to allow the researcher to assess transfection efficiency by fluorescence microscopy.
No product specific background information is currently available for this product.
Application References
- Dai, Y. et al. (2004) A Bcr/Abl-independent, Lyn-dependent form of imatinib mesylate (STI-571) resistance is associated with altered expression of Bcl-2. Journal Of Biological Chemistry 279, 34227-34239. This article references the use of SignalSilence® Control siRNA (Fluorescein Conjugate) in the following applications: siRNA
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 6200 SignalSilence® ILK1 siRNA Kit (Human Specific)
- 6202 SignalSilence® ILK1 siRNA (Human Specific)
- 6210 SignalSilence® Akt siRNA Kit
- 6211 SignalSilence® Akt siRNA
- 6230 SignalSilence® p53 siRNA Kit (Human Specific)
- 6231 SignalSilence® p53 siRNA (Human Specific)
- 6240 SignalSilence® Chk1 siRNA Kit (Human Specific)
- 6241 SignalSilence® Chk1 siRNA (Human Specific)
- 6250 SignalSilence® PTEN siRNA Kit (Human Specific)
- 6251 SignalSilence® PTEN siRNA (Human Specific)
- 6260 SignalSilence® NF-κB p65 siRNA Kit (Human Specific)
- 6261 SignalSilence® NF-κB p65 siRNA (Human Specific)
Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
