Product Pathways - Chromatin Regulation / Epigenetics
ACF1 Antibody #6255
|6255S||100 µl (10 western blots)||---||In Stock||---|
|6255||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
ACF1 Antibody recognizes endogenous levels of total ACF1 protein (isoforms 1 and 2).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met864 of human ACF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
The mammalian imitation SWI (ISWI) complexes are characterized by two ATPase subunits: Snf2h and Snf2l (1). Snf2h interacts with ATP-utilizing chromatin assembly and remodeling factor 1 (ACF1) to comprise the ACF chromatin-remodeling complex (1). ACF1 (BAZ1A) has distinct roles in development (2), regulation of chromatin structure (3), and DNA damage response (4,5). Different developmental stages dictate the expression of ACF1 in Drosophila, and alterations in ACF1 expression during Drosophila development leads to deviation from normal chromatin organization (2). ACF1 functions in heterochromatin formation during development and is involved in the initial establishment of diversified chromatin structures. In vivo studies demonstrate that heterochromatin protein 1 (HP1) binding to methylated lysine 9 of histone H3 is enhanced by the interaction of ACF1 with chromatin (6). Chromatin-remodeling factors are required during DNA damage in order to allow signaling molecules and damaging enzymes to access the site (4). Depletion of hACF1 increases apoptosis and vulnerability to radiation and compromises G2/M arrest activated in response to X-ray and UV exposure (4). Depletion of ACF1 also sensitizes cells to DNA double-stranded breaks (DSBs) and impairs DNA repair (5). Specifically, accumulation of Ku at DSBs sites may depend on the presence of ACF1 (5).
- Saladi, S.V. and de la Serna, I.L. (2010) Stem Cell Rev 6, 62-73.
- Chioda, M. et al. (2010) Development 137, 3513-22.
- Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
- Sánchez-Molina, S. et al. (2011) Nucleic Acids Res 39, 8445-56.
- Lan, L. et al. (2010) Mol Cell 40, 976-87.
- Eskeland, R. et al. (2007) Mol Cell Biol 27, 453-65.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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