Cell Signaling Technology

Product Pathways - Angiogenesis

ETS-1 Antibody #6258

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 52 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ETS-1 Antibody recognizes endogenous levels of total ETS-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser179 of human ETS-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ETS-1 Antibody.

Background

ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence (ETS) family of transcription factors that share a unique and highly conserved DNA binding domain (1). ETS-1 plays important roles in vascular development and angiogenesis (2), and vascular inflammation and remodeling (3). The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules (4-6). In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells (7). ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity (8), phosphorylation at its Thr38 site by Ras activates ETS-1 (9).

  1. Bartel, F.O. et al. (2000) Oncogene 19, 6443-54.
  2. Sato, Y. (2001) Cell Struct Funct 26, 19-24.
  3. Oettgen, P. (2006) Circ Res 99, 1159-66.
  4. Dube, A. et al. (1999) Circ Res 84, 1177-85.
  5. Lelièvre, E. et al. (2000) Oncogene 19, 2438-46.
  6. Ghosh, S. et al. (2012) J Biol Chem 287, 15001-15.
  7. Verschoor, M.L. et al. (2010) PLoS One 5, e13565.
  8. Pognonec, P. et al. (1988) EMBO J 7, 977-83.
  9. Yang, B.S. et al. (1996) Mol Cell Biol 16, 538-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products