Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

COUP-TFI (D4H2) Rabbit mAb #6364

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 47 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

COUP-TFI (D4H2) Rabbit mAb recognizes endogenous levels of total COUP-TFI protein. The antibody does not cross-react with COUP-TFII protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln165 of human COUP-TFI protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using COUP-TFI (D4H2) Rabbit mAb.

Background

Chicken ovalbumin upstream promoter transcription factor (COUP-TF) belongs to the NR2 subfamily of the nuclear hormone receptor family (1). COUP-TFI and COUP-TFII are two of the well-characterized members in the NR2 subfamily. These two members are highly conserved in their two zinc-finger DNA binding domains (DBD) and the ligand binding domain (LBD), and function as repressors or activators of downstream target genes to regulate different biological processes (1-3). COUP-TFI and II bind to 5'-AGGTCA-3' motif palindromes, either directly or indirectly, through heterodimer formation with other proteins (e.g. RXRs) to regulate downstream target gene expression (4,5). COUP-TFI is involved in neuronal development, tissue patterning, and differentiation (6-8). COUP-TFII has been shown to be involved in angiogenesis, glucose homeostasis, and mesenchymal cell commitment (9-12).

  1. Tsai, S.Y. and Tsai, M.J. (1997) Endocr Rev 18, 229-40.
  2. Park, J.I. et al. (2003) Keio J Med 52, 174-81.
  3. Lin, F.J. et al. (2011) Endocr Rev 32, 404-21.
  4. Cooney, A.J. et al. (1992) Mol Cell Biol 12, 4153-63.
  5. Montemayor, C. et al. (2010) PLoS One 5, e8910.
  6. Zhou, C. et al. (2001) Genes Dev 15, 2054-9.
  7. Alfano, C. et al. (2011) Development 138, 4685-97.
  8. Faedo, A. et al. (2008) Cereb Cortex 18, 2117-31.
  9. Li, L. et al. (2009) Cell Metab 9, 77-87.
  10. Pereira, F.A. et al. (1999) Genes Dev 13, 1037-49.
  11. Qin, J. et al. (2010) Cancer Res 70, 8812-21.
  12. Xie, X. et al. (2011) Proc Natl Acad Sci U S A 108, 14843-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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